Detail Information
The Opal™ 7 Solid Tumor Immunology Kit enables phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections.
Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).
The Opal™ 7 Solid Tumor Immunology Kit includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections using 6-plex fluorescent immunohistochemistry:
- Primary antibodies
- Antibody diluent
- Fluorescent IHC detection reagents
- Spectral DAPI
- Antigen retrieval buffers
This kit was configured and validated for multispectral imaging on the Vectra and Mantra Systems. It contains enough reagents for 6-plex immunostain of 50 slides.
- Optimized panel for assessment of immune cells in intact FFPE tumor tissue.
- Shortest path to results
- Part of the Phenoptics™ workflow for immunophenotyping of solid tumors
Specifications
| Assay Target | CD4, CD8, CD20, FOXP3, CD68, panCK |
|---|---|
| Automation Compatible | No |
| Detection Method | MSI, Fluorescence |
| Fluorophore | Opal 520,Opal 540,Opal 570,Opal 620,Opal 650,Opal 690 |
| Product Brand Name | Opal |
| Quantity in a Package Amount | 1.0 per pack |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Unit Size | 50 slides |
Resources, Events & More
Brochure
Brochure: Phenoptics Portfolio
Case Study
Case Study: Improving Cancer Vaccine Efficacy
E-book
Download Phenoptics Research Solutions E-book
Flyer
Flyer: Opal Multiplex IHC Detection Kits
Product Information Bulletin
Product Info: Let's Rid the World of Ribbons - Infographic
Scientific Paper
Publication: NEJM: Mutations Associated with Acquired Resistance to PD-1 Blockade in Melanoma
Publication: Novartis Genoptix Shows Multiplexed IHC Test Better Predicts Immunotherapy Response
Publication: NEJM: PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma
Scientific Poster
Poster: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining
Poster: Automate Your Complete Cancer Research Workflow with Phenoptics™
Support Pages
Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs
Webinars
Webinar: Understanding the Tumor Immune Composition Using Multiplexed Quantitative Analyses
Webcast: The Beginning of the End - Immunoprofiling Coming of Age
Webinar: Developing and Validating a Multiplexed IHC/Immunofluorescent Assay for Cancer Immunology
Material Safety Data Sheets