The Opal™ 7 Solid Tumor Immunology Kit enables phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections.
Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).
The Opal™ 7 Solid Tumor Immunology Kit includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections using 6-plex fluorescent immunohistochemistry:
- Primary antibodies
- Antibody diluent
- Fluorescent IHC detection reagents
- Spectral DAPI
- Antigen retrieval buffers
This kit was configured and validated for multispectral imaging on the Vectra and Mantra Systems. It contains enough reagents for 6-plex immunostain of 50 slides.
- Optimized panel for assessment of immune cells in intact FFPE tumor tissue.
- Shortest path to results
- Part of the Phenoptics™ workflow for immunophenotyping of solid tumors
|Assay Target||CD4, CD8, CD20, FOXP3, CD68, panCK|
|Detection Method||MSI, Fluorescence|
|Fluorophore||Opal 520,Opal 540,Opal 570,Opal 620,Opal 650,Opal 690|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Shipping Condition||Blue Ice|
|Unit Size||50 slides|
Resources, Events & More
Product Information Bulletin
Publication: Linch, Stefanie N., et al. "Combination OX40 agonism/CTLA-4 blockade with HER2 vaccination reverses T-cell anergy and promotes survival in tumor-bearing mice." Proceedings of the National Academy of Sciences 113.3 (2016): E319-E327
Publication: Woods, Katherine, et al. "Mismatch in epitope specificities between IFNγ inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma." Journal for immunotherapy of cancer 4.1 (2016)
Material Safety Data Sheets